WebThe homologous recombination fragment was inserted into the integration vector pMUTIN-GFP+, which could integrate into chromosome by a single recombination event, to construct knockout plasmid.

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WebJan 1, 2016 · Transformants were PCR confirmed using pMUTIN-GFP gene specific primers ( Table S1) that were designed from gfp gene sequences for amplification of 359 bp. PCR was performed in GoTaq PCR kit of Promega (GoTaq Buffer-1X, MgCl 2 – 1.5 mmol l −1, dNTPs – 200 μmol l −1, Forward/Reverse primers – 10 pmol each, DMSO – 6%, Taq … WebThe mechanism of pBacTag Tagging Vectors is illustrated with pBacTag-GFP+ as example. This vector can be used for creating a GFP+ fusion protein from any chromosomally located gene of interest, by fusing a gfp+ tag to the chosen gene. In this example the gene of interest is named orf2. It is part of an operon, including three genes domino\u0027s pizza roanoke rapids nc

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Web(pMutin-GFP+) Ap: GM2163(pMutin-GFP+) Shipping Conditions: LB agar with ampicillin (100 µg/ml) Growth Conditions: LB agar with ampicillin (100 µg/ml) Temperature: 37: References: Kaltwasser M, Wiegert T, Schumann W. Construction and application of epitope- and green fluorescent protein-tagging integration vectors for Bacillus subtilis. Appl ... WebMay 7, 2010 · We have constructed a set of plasmids that allow expression, from their native chromosomal loci, of Staphylococcus aureus proteins fused to one of four different fluorescent proteins (green fluorescent protein [GFP], cyan fluorescent protein [CFP], yellow fluorescent protein [YFP], and mCherry), using two different resistance markers … WebThe invention discloses bacillus gene traceless knockout/enter plasmid, method and kit, contain in the genome of the bacillus gene traceless knockout/enter plasmid：Only a kind of resistant gene of... qrljacking