Phospho-egfr
WebDetection of Phospho-EGFR in EGF-treated A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was untreated (open histogram) or treated for 5 minutes with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG, filled histogram) then stained with Human Phospho--EGFR (Y1068) Monoclonal Antibody (Catalog # MAB3570), … WebThe Human EGFR (Phospho) [pY1068]) ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of EGFR (Phospho) [pY1068]) in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant human EGFR (Phospho) [pY1068]. Principle of the method
Phospho-egfr
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WebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1068) + pan EGFR. Number of Targets Detected: 2 Species Detected: Human Mouse Compatible Sample Types: Cell Lysates Tissue Lysates Design Principle: Sandwich-based Method of Detection: Colorimetric Quantitative/Semi-Quantitative: Semi-Quantitative Solid Support: WebMar 21, 2024 · EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer.
WebAug 1, 2007 · Antibodies against the following proteins were used: AIB1, c-Cbl, SHP-1, PTP-1B, and Grb2 (BD Transduction); EGFR (1005); phosphorylated EGFR (phospho-EGFR; Tyr 845, Tyr 992, Tyr 1045, Tyr 1068, Tyr 1173, and Ser 1046 /Ser 1047 ), phosphorylated AKT (phospho-AKT; Ser 473 ), phosphorylated HER2 (phospho-HER2; Tyr 1221 /Tyr 1222 ), … WebNational Center for Biotechnology Information
WebApr 12, 2024 · EGFR levels were slightly higher in resistant cells, but phospho-EGFR (pEGFR) levels were significantly lower in untreated resistant cells, which reduced further upon … WebTHUNDER™ Phospho-EGFR (Y1068) TR-FRET Cell Signaling Assay Kit Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EGFR (Y1068) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. KIT-EGFRP-100 Size Components Eu-phospho …
WebPhospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb detects endogenous EGF receptors only when phosphorylated at Tyr1173. This antibody may cross-react with other activated …
WebJun 30, 2016 · The level of phosphorylated EGFR in the untreated H1975 cells (EGFR mutations L858R; T790M) was higher than that of H292 cells (EGFR WT) due to the activating mutation in EGFR. Addition of EGF to H1975 cells resulted in a smaller induction of phosphorylated EGFR compared with the magnitude of induction observed with H292 … darling harbour fireworks 2022WebPhospho-EGFR (Tyr1173) Assay. Increased signal is observed with the titration of pEGFR positive cell lysate. The Phospho-EGFR (Tyr1173) Assay provides a quantitative measure … bismarck foundedWebThe phospho-EGFR assay kit is designed for the cell-based quantitative detection of Tyr1068 phosphorylation on EGFR. Mutations that lead to an overexpression or hyperactivity of EGFR, also known as ErbB1 or HER1, are associated with a number of cancers, such as lung, colorectal, prostate, glioblastoma cancer, etc. darling harbour fireworks ticketsWebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material. darling harbour fireworks saturdayWebPhosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of EGFR at Tyr845. The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of ... bismarck frosty cupWebJun 17, 2009 · To compare the site-specific phosphorylation pattern to total phosphorylation of EGFR we incubated cells with ligand on ice for 1 h followed by incubation at 37°C for 5 or 15 min (data not shown and Figure S6C), and cell lysates were subjected to IP of EGFR. The immunoprecipitates were analysed by western blotting using EGFR … bismarck forecast 7 dayWebDec 8, 2015 · Phosphorylation sites in the EGFR tail are suboptimal but are robustly phosphorylated by EGFR, probably because of high local concentrations around the … bismarck frosty cup soccer tournament