WebProduct Name: Phosphate-buffered saline (DPBS, 1X), Dulbecco's formula, without calcium, without magnesium Catalog Number: J67802.K2 Product Specification Appearance (Color): Clear, colorless Composition: 137.9 mM Sodium Chloride, 1.47 mM Potassium Phosphate Monobasic, 2.67 mM Potassium Chloride, 8.09 mM Sodium Phosphate Dibasic pH: 7.4 ± … WebCarefully wash sample with DPBS to remove nonbound probe. 3. Add a sufficient volume of Hoechst 33342 Working Solution to completely cover the sample. Place aluminum foil over the sample to protect it from light and incubate at room temperature for 5-10 minutes. Optimize incubation time to minimize signal bleeding through to other channels. 4.
Fisher Scientific SH3002802 - McKesson Medical-Surgical
WebDulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. ... Fisher Scientific BVBA - Havenlaan 86c Av. Du Port - Bus/Boîte 204 1000 - BRUSSEL/BRUXELLES S ... WebJan 21, 2024 · α-Synuclein (αSyn) is an important player in Parkinson’s disease (PD) pathogenesis. The aggregation of αSyn is mainly formed in the cytoplasm, whereas some αSyn accumulation has also been found in the nuclei of neurons. how many people can twerk
DPBS (10X), no calcium, no magnesium from Thermo Fisher …
Webserum-free and serum supplemented systems. TrypLE™ products are formulated in DPBS/1 mM EDTA, are room-temperature stable, and are convenient to use. Contents and storage Product Cat. No. Amount Storage Shelf life[1] TrypLE™ Select Enzyme (1X), no phenol red 12563011 12563029 100 mL 500 mL 15°C to 30°C; Protect from light 24 months WebThe next day, the cells were washed 3 × 5 min with DPBS and incubated 1 h in the dark, RT, with 200 μL fluorescently labeled secondary antibodies in DPBS with 1% BSA, in a ratio of 1:1000. After washing the cells three times with DPBS, the cell nuclei were stained with a DAPI (#32670, Sigma-Aldrich, St. Louis, MO, USA) in distilled water (1: ... WebFisher Scientific: Country of Origin: Unknown: Application: Cell Culture Reagent: Chemical Name: Dulbecco’s Phosphate Buffered Saline (DPBS) Concentration Level: 10X / pH 7.0 … how many people can stay in a 1 bedroom